The pub, which is owned by James and Kate Mackenzie, picked up the honour at a special awards lunch held at Hermitage Rd in Hitchin, Hertfordshire, on Monday (26 January).
It overcame two other finalists to win the award, which is sponsored by leading wine merchant Berkmann Wine Cellars.
The pub had further success, when it was placed third in the Gastropub Awards Top 50.
Organised by leading pub industry magazine the Publican’s Morning Advertiser, the Budweiser Budvar Top 50 Gastropub Awards are judged by pub guide editors and business journalists.
The awards were hosted by BBC Two Food & Drink presenter and two-Michelin starred chef Tom Kerridge.
The Sportsman, in Seasalter, Kent, was crowned Budweiser Budvar Top 50 Gastropub of the Year.
In keeping with the Pipe & Glass’s all-encompassing food offer, the pub’s wine list provides a variety of wines for each price point. Bottles range from £17 to £100, but around half of the 100-strong wine list is kept under the £30 mark.
To help customers match wine with food, and to guide them away from the normal regional sections, the list is grouped under food categories such as ‘fish/shellfish’ and ‘poultry and game’.
Jo Bruce, managing editor at the Publican’s Morning Advertiser, said: “The Pipe & Glass is an outstanding gastropub that has made a great impression with its comprehensive wine offer.
“The pub regularly holds wine dinners with producers and suppliers. It even offers an own label house wine.
“Grouping wines under complementary food categories is a clever innovation that other pubs can learn from.”